An experiment was conducted to investigate the influence of vitamin C and E blood metabolites and performance of calves from one day old up to 42 days of ages. In this trial 24 one day old calves were randomly allocated to 6 experimental groups (4 calves per each treatment). Experimental group were 1.control (no supplemented vitamin), 2. Vitamin C (3g/day), 3. Supplemented vitamin C with following order (first and second weeks of age (3g/day), third and fourth weeks of ages (2g/day), fifth and six week of ages (1g/day)), 4. Combination of vitamin E (1g/day) and vitamin C (2g/day), 5. Supplemented with vitamin E (2g/day), 6. Supplemented vitamin E with following order (first and second weeks of ages (0.6g/day), third and fourth weeks of ages (0.9 g/day) and finally (1.2 g/day during fifth and six week of ages. Blood samples were taken from jugulars vein of each animal at the 3, 14, 28 and 42 day of ages. Serum was assayed for total protein, glucose, PCV, hemoglobin, the lymphocyte to neutrophil ratio, ratio of albumin to globulin and Gama globulin. The Gama globulin concentration 28 and 42 days of ages of calves, among of treatment were statistically significant (p<0.05). The calves that were fed with treatment 3 had higher gamaglobulin than the other groups. The ratio of albumin to globulin at 28 days of ages was significantly different among groups(p<0.05), by consideration of that calves given vitamin supplements in group 5,3,2 the ratio of albumin to globulin were higher than other treatment. However there were no significant differences between these three groups (p>0.05). Total serum protein, albumin, glucose, hemoglobin concentration, PCV and the ratio of lymphocyte to neutrophil did not an affect by treatment (p>0.05). Dietary contained vitamin supplement had statistically significant effect on daily weight again (p<0.01). Animal which treated by vitamins had shown higher growth rate than other group